Botanica Pacifica. A journal of plant science and conservation 2025. Preprint
Article first published online: 29 JAN 2025 | DOI: 10.17581/bp.2025.14106
Effects of pH of a nutrient medium on parameters of growth and rhizogenesis of wild-type blue honeysuckle (Lonicera edulis (Turcz. ex Herder) Turcz. ex Freyn) in in vitro culture
Anna A. Erst 1,2
,
Irina G. Boyarskikh 1 ,
Evgeny V. Banaev 1 &
Maria A. Tomoshevich 1 1 Central Siberian Botanical Garden SB RAS, Novosibirsk, Russia
2 Ningbo Osaki Biotech Co., Ltd., Ningbo, China
Under natural conditions, species of blue honeysuckles grow on soils with a wide range of pH. In this work, for the first time under artificial in vitro conditions, the influence of nutrient medium pH of 3.8 to 8.3 on the viability and growth and development parameters was studied in a species Lonicera edulis from the bluehoneysuckle subsection. It was found that nutrient medium pH does not affect the propagation coefficient of Lonicera edulis; however, more alkaline media caused shortening of its shoots by reducing the length of internodes. Our data suggested that pH affects the rooting of in vitro microshoots of the species under study. Two optima for the rhizogenesis were found: pH 3.8–4.3 (56.5–60.1 % rooting capacity) and pH 6.3–6.8 (53.8–61.1 %). Meanwhile, the height of the shoots and the number of leaves at the rooting stage did not significantly differ among pH values of the medium. Thus, these in vitro culture experiments confirm the tolerance of Lonicera edulis to a wide pH range.
Эрст А.А., Боярских И.Г., Банаев Е.В., Томошевич М.А. Влияние рН питательной среды на параметры роста и ризогенеза дикорастущей голубой жимолости (Lonicera edulis (Turcz. ex Herder) Turcz. ex Freyn) в культуре in vitro. В естественных условиях голубые жимолости произрастают на почвах с широким диапазоном pH. В данной работе впервые в искусственных условиях in vitro исследовано влияния pH питательной среды от 3.8 до 8.3 на жизнеспособность и показатели роста и развития Lonicera edulis. Отмечено, что pH питательной среды не оказывает влияния на коэффициент размножения Lonicera edulis, однако более щелочные среды вызывают укорочение побегов за счет сокращения длины междоузлий. Полученные данные свидетельствуют о том, что значение pH влияет на укореняемость in vitro микропобегов. Выявлено два оптимума для укоренения: pH 3.8–4.3 (56.5–60.1 %) и pH 6.3–6.8 (53.8–61.1 %). При этом высота побегов и количество листьев на стадии укоренения достоверно не различались при различных значениях pH среды. Проведенные в культуре in vitro исследования подтверждают устойчивость Lonicera edulis к широкому диапазону pH.
Keywords: blue honeysuckle, in vitro propagation, pH range, tolerance, голубые жимолости, in vitro размножение, диапазон pH, толерантность
References
Abdulaha-Al Baquy, M., J.Y. Li, C.Y. Xu, K. Mehmood & R.K. Xu 2017. Determination of critical pH and Al concentration of acidic Ultisols for wheat and canola crops. Solid Earth 8:149-159. CrossRef
Banaev, E.V., A.A. Erst, E.P. Khramova, M.A. Tomoshevich & T.M. Shaldaeva. 2024. In vitro culture of Atraphaxis frutescens (L.) K. Koch: effects of D-mannitol and pH on a propagation coefficient, secondary-metabolite levels, and antiradical activity. Plant Cell, Tissue and Organ Culture 156(2):46. CrossRef
Bhatia, P. & N. Ashwath 2005. Effect of medium pH on shoot regeneration from the cotyledonary explants of tomato. Biotechnology 4:7-10. CrossRef
Boyarskikh, I.G. & T.I. Siromlya 2022. Macro- and microelement composition of blue honeysuckle and oakleaved spirea in cenopopulations of Altai Mountains under conditions of geochemical anomaly. Khimiya Rastitel'nogo Syr'ya 4:211-220 (in Russian). [Боярских И.Г., Сиромля Т.И. 2022. Макро- и микроэлементный состав жимолости синей и спиреи дубравколистной в ценопопуляциях Горного Алтая в условиях геохимической аномалии // Химия растительного сырья. № 4. С. 211-220].
Boyarskikh, I.G. & T.I. Syromlya 2022. Features of the accumulation of macro- and microelements in the organs of Lonicera caerulea L. plants and their effect on the reproductive characteristics in the zone of geological disjunctive disturbances (the Altai Mountains, the Kyzyl-Yaryk River). Khimiya v interesah ustojchivogo razvitiya 30:341-353 (in Russian). [Боярских И.Г., Сиромля Т.И. 2022. Особенности накопления макро- и микроэлементов в органах Lonicera caerulea L. и их влияниерепродуктивные характеристики в зоне геологических дизъюнктивных нарушений (Горный Алтай, р. Кызыл-Ярык) // Химия в интересах устойчивого развития. № 30. С. 1-13]. CrossRef
Boyarskikh, I.G., A.I. Syso & T.I. Siromlya 2018. Mineral composition of Lonicera caerulea phytomass in contrasting geochemical environments. Khimiya Rastitel'nogo Syr'ya 3:129-138 (in Russian). [Боярских И.Г., Сысо А.И., Сиромля Т.И. 2018. Особенности минерального состава Lonicera caerulea в контрастных геохимических условиях // Химия растительного сырья. № 3. С. 129-138]. CrossRef
Brown, D.C.W., D.W.M. Leung & T.A. Thorpe. 1979. Osmotic requirement for shoot formation in tobacco callus. Physiologia Plantarum 46:36-41. CrossRef
Cuce, M., T.Bekircan & A. Sokmen 2016. Effect of medium pH on shoot regeneration of three Vaccinium species naturally growing in Turkish Flora. Turkish Journal of Life Sciences 1:52-58.
Dawson, J.K. 2017. Concentration and content of secondary metabolites in fruit and leaves of Haskap (Lonicera caerulea L.). PhD thesis. Saskatoon, Canada.
Dziedzic, E. 2008. Propagation of blue honeysuckle (Lonicera caerulea var. kamtschatica Pojark.) in in vitro culture. Journal of Fruit and Ornamental Plant Research 16:93-100.
Erst, A.A. & A.V. Karakulov 2023. Drought tolerance of ornamental poplar forms cultured in vitro. Contemporary Problems of Ecology 16:339-345. CrossRef
Erst, A.A., A.A. Petruk, A.S. Erst, D.A. Krivenko, N.V. Filinova, S.Y. Maltseva, M.S. Kulikovskiy & E.V. Banaev 2022. Optimization of biomass accumulation and production of phenolic compounds in callus cultures of Rhodiola rosea L. using design of experiments. Plants 11:124. CrossRef
Figiel-Kroczyńska, M., M. Krupa-Małkiewicz & I. Ochmian 2022. Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum. Acta Scientiarum Polonorum Hortorum Cultus 21:25-37. CrossRef
Fira, A., D. Clapa, V. Cristea & C. Plopa 2014. In vitro propagation of Lonicera kamtschatica. Agricultura - Ştiinţă şi practică 1-2:90-99.
Gagnon, A. 2015. La Camerise: Guide de Production; Publication 15-0031. Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec (MAPAQ), Québec, QC, Canada.
George, E.F., M.A. Hall & G.J.D. Klerk 2008. The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems. In: Plant propagation by tissue culture, 3rd edn volume 1, the background (E.F. George et al., eds), pp. 115-173, Springer, Dordrecht, The Netherlands. CrossRef
Glinushkin, A.P., S.V. Akimova, E.A. Nikulina, N.V. Tsirulnikova, V. Kirkach, V.P. Kalinitchenko, A. Radzhabov, E. Radkevich, L. Marchenko, A. Solovyov, ... & V. Indolov 2023. Preliminary study: micropropagation using five types of chelated iron and the subsequent acclimation of blue honeysuckle (Lonicera caerulea var. kamtschatica Sevast.). Forests 14(4):821. CrossRef
Golba, M., A.Sokol-Letowska & A.Z. Kucharska 2020. Health properties and composition of honeysuckle berry Lonicera caerulea L. An update on recent studies. Molecules 25(3):749. CrossRef
Jurikova, T., O. Rop, J. Mlcek, J. Sochor, S. Balla, L. Szekeres, A. Hegedusova, J. Hubalek, V. Adam & R. Kizek 2012. Phenolic profile of edible honeysuckle berries (genus Lonicera) and their biological effects. Molecules 17:61-79. CrossRef
Kerley, S.J. 2000. The effect of soil liming on shoot development, root growth, and cluster root activity of white lupin. Biology and Fertility of Soils 32:94-101. CrossRef
Kolbanova, E.V. 2023. Clonal micropropagation of Belarusian varieties of blue honeysuckle (Lonicera caerulea L. var. kamtschatica) Weight. Izvestiya Nacional'noi akademii nauk Belarusi. Seriya agrarnyh nauk 61(1):34-47 (in Russian). [Колбанова Е.В. 2023. Клональное микроразмножение белорусских сортов жимолости синей (Lonicera caerulea L. var. kamtschatica) // Известия Национальной академии наук Беларуси. Серия аграрных наук. Т. 61, № 1. С. 34-47]. CrossRef
Kovačević, B., D. Miladinović, M. Katanić, Z. Tomović & S. Pekeč 2013. The effect of low initial medium pH on in vitro white poplar growth. Glasnik Sumarskog fakulteta 108:67-80. CrossRef
Kuklina, A.G. & L.I. Vozna 1988. Soil conditions of blue honeysuckle habitats. Byulleten' Glavnogo botanicheskogo sada 147:41-45 (in Russian). [Куклина А.Г., Возна Л.И. 1988. Почвенные условия местообитаний жимолости голубой // Бюллетень Главного ботанического сада. Вып. 147. С. 41-45].
Leifert, C., S. Pryce, P.J. Lumsden & W.M. Waites 1992. Effect of medium acidity on growth and rooting of different plant species growing in vitro. Plant Cell, Tissue and Organ Culture 30:171-179. CrossRef
Marcelina, K. & O. Ireneusz 2014. Propagation of blue honeysuckles (Lonicera caerulea L.) in in vitro culture. Journal of Basic and Applied Sciences 10:164-169. CrossRef
Marhold, K., J. Kučera, T.G. Aleksandrova, T.V. Alexeeva, E.A. Andriyanova, E.V. Banaev, A.A. Bobrov, E.V. Boltenkov, E.A. Bondarevich, I.G. Boyarskikh, ... & E.Y. Zykova 2022. IAPT chromosome data 38/2. Taxon 71(6):1353-1360, E13-E33. CrossRef
Minaeva, V.G. 1991. Medicinal plants of Siberia. Nauka, Novosibirsk, Russia, 431 pp. (in Russian). [Минаева В.Г. 1991. Лекарственные растения Сибири. Новосибирск: Наука. 431 с.].
Murashige, T. & F. Skoog 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15(2):473-497. CrossRef
Naik, P.M., S.H. Manohar, N. Praveen & H.N. Murthy 2010. Effects of sucrose and pH levels on in vitro shoot regeneration from leaf explants of Bacopa monnieri and accumulation of bacoside A in regenerated shoots. Plant Cell, Tissue and Organ Culture 100:235-239. CrossRef
Orlova, N., E. Raeva-Bogoslovskaya, A. Miroshnichenko & O. Molkanova 2022. Some clonal micropropagation features of Lonicera caerulea L. cultivars in vitro. BIO Web of Conferences 47:04005. CrossRef
Plekhanova, M.N. 2000. Blue honeysuckle (Lonicera caerulea L.) - a new commercial berry crop for temperate climate: genetic resources and breeding. Acta Horticulturae 538:159-164. CrossRef
Plekhanova, M.N. 2006. On the species composition of the blue honeysuckle subsection Lonicera subsect. Caerulea (Fam. Caprifoliaceae). Trudy po prikladnoj botanike, genetike i selektsii 162:59-70 (in Russian). [Плеханова М.Н. 2006. О видовом составе подсекции голубых жимолостей Lonicera subsect. Caerulea (Fam. Caprifoliaceae) // Труды по прикладной ботанике, генетике и селекции. Т. 162. С. 59-70].
Poyarkova, A.I. 1958. Genus Lonicera L. In: Flora of the USSR, vol. 23 (B.K. Shishkin, ed.), pp. 467-573, Izdatel'stvo AN SSSR, Moscow, Leningrad (in Russian). [Пояркова А.И. 1958. Род Lonicera L. // Флора СССР / под ред. Б.К. Шишкина. Москва; Ленинград: Издательство АН СССР. Т. 23. С. 467-573].
Rai, M.K., R.K. Kalia, R. Singh, M.P. Gangola & A.K. Dhawan 2011. Developing stress tolerant plants through in vitro selection - An overview of the recent progress. Environmental and Experimental Botany 71:89-98. CrossRef
Rehder, A. 1903. Synopsis of the genus Lonicera L. Missouri Botanical Garden Annual Report 1903 (1903):27-232. CrossRef
Renata, M. 2001. Study of vegetative, growing and economic character of genus Lonicera subsect. Caeruleae Rehd. In: 9th international conference of horticulture: proceedings: September 3th-6th 2001 (P. Salaš, ed.), Lednice, Czech Republic.
Sahu, M., S. Maurya & Z. Jha 2023. In vitro selection for drought and salt stress tolerance in rice: an overview. Plant Physiology Reports 28:8-33. CrossRef
Sedlák, J. & F. Paprštein 2007. In vitro propagation of blue honeysuckle. Horticultural Science 34(4):129-131. CrossRef
Sharma, A. & H-J. Lee 2021. Lonicera caerulea: An updated account of its phytoconstituents and health-promoting activities. Trends in Food Science & Technology 107:130-149. CrossRef
Skvortsov, A.K. & A.G. Kuklina 2002. Blue honeysuckle: Botanical study and cultural perspectives in central Russia. Nauka, Moscow, 160 pp. (in Russian). [Скворцов А.К., Куклина А.Г. 2002. Голубые жимолости: Ботаническое изучение и перспективы культуры в средней полосе России. Москва: Наука, 160 с.].
Tremblay, C., A. Deslauriers, J. Lafond, J. Lajeunesse & M.C. Paré 2019. Effects of soil pH and fertilizers on Haskap (Lonicera caerulea L.) vegetative growth. Agriculture 9(3):56. CrossRef
|
|