Botanica Pacifica. A journal of plant science and conservation 2025. Preprint
Article first published online: 27 JAN 2025 | DOI: 10.17581/bp.2025.14105
The effect of elevation gradient on cytogenetic characteristics of Plantago major L. (Plantaginaceae)
Marianna B. Malaeva 1
,
Nina V. Reutova 1 ,
Fatima R. Dreeva 1 ,
Tatiana V. Reutova 1 &
Petimat M. Dzhambetova 2 1 Kabardino-Balkar Scientific Center RAS, Nalchik, Russia
2 Chechen State University, Department of Cell Biology and Microbiology, Grozny, Russia
We aimed to study the effect of elevation gradient on the cytogenetic characteristics of Plantago major L. We determined the mitotic index, the frequency of chromosomal aberrations, micronuclei and chromosome numbers in the cells of the apical root meristem of the seed progeny of plants inhabit altitudes of 200, 600, 2000 and 2700 m above sea level. The frequency of chromosomal aberrations increased at altitudes more than 2000 m above sea level, which indicates the presence of a genetic effect. There was no similar increase in the frequency of micronuclei in interphase cells. There were no trends in cell proliferation (mitotic index) and the ratio of mitosis phases in accordance with the elevation gradient. Plants from populations inhabit different altitudes are diploids. No changes in chromosome numbers along the altitude gradient were detected.
Малаева М.Б., Реутова Н.В., Дреева Ф.Р., Реутова Т.В., Джамбетова П.М. Влияние высотной поясности на цитогенетические характеристики Plantago major L. (Plantaginaceae). Был изучен ряд цитогенетических характеристик подорожника большого (Plantago major L.) в высотном градиенте под влиянием экстремальных условий высокогорья. Мы определяли митотический индекс, частоту хромосомных аберраций, микроядер и хромосомные числа в клетках апикальной корневой меристемы семенного потомства растений, обитающих на высотах 200, 600, 2000 и 2700 м над уровнем моря. У растений, обитающих на высотах более 2000 м над уровнем моря, возрастает частота хромосомных аберраций. Аналогичного повышения частоты микроядер в интерфазных клетках не выявлено. Изменений в пролиферации клеток (митотический индекс) и соотношении фаз митоза в соответствии с высотным градиентом не выявлено. Растения из популяций, обитающих на разных высотах, являются диплоидами. Изменения плоидности по высотному градиенту не выявлено.
Keywords: Central Caucasus, altitude gradient, mitotic index, micronuclei, chromosomal aberrations, polyploidy, Центральный Кавказ, высотный градиент, митотический индекс, микроядра, хромосомные аберрации, полиплоидия
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