Botanica Pacifica. A journal of plant science and conservation 2025. Preprint
Article first published online: 11 MAR 2025 | DOI: 10.17581/bp.2025.14111
In vitro propagation of herbaceous species of the Aristolochia genus
Olga V. Nakonechnaya
,
Elizabeth P. Yusupova ,
Vlada V. Volkonskaya &
Irina V. Gafitskaya Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RAS, Vladivostok, Russia
This report provides the results of microclonal propagation of nine herbaceous species of the genus Aristolochia from to the sections Diplolobus and Gymnolobus. Each of the species has medicinal properties. For the cultivation, Murashige and Skoog medium was used, a hormone-free nutrient medium with a half concentration of macro- and micronutrients and iron chelate. Aristolochia clematitis was characterized by the highest growth rate, and A. baetica by the lowest rate. Explants of A. rotunda showed the highest potential for propagation (with the propagation coefficient equal to 7.5), and A. gigantea had the lowest potential (3.32), which was associated with the growth characteristics of the species under study. The obtained results of successful propagation allow simplification of the existing protocols. The use of the nutrient medium of modified composition is expected to reduce material and labor costs in implementation of programs for conservation of valuable medicinal plant resources.
Наконечная О.В., Юсупова Е.П., Волконская В.В., Гафицкая И.В. Размножение in vitro травянистых видов рода Aristolochia. Представлены результаты по микроклональному размножению 9 травянистых видов рода Aristolochia, относящихся к секциям Diplolobus и Gymnolobus. Каждый из видов обладает лекарственными свойствами. Для культивирования использовали безгормональную питательную среду с половинной концентрацией макро- и микроэлементов и хелата железа по Мурасиге и Скугу. Показано, что наибольшая скорость роста характерна для Aristolochia clematitis, наименьшая для A. baetica. Наибольшим потенциалом к размножению обладали экспланты A. rotunda (коэффициент размножения равен 7,5 шт.), наименьшим – A. gigantea (3,32 шт.), что связано с особенностями роста каждого исследованного вида. Полученные результаты успешного размножения позволили упростить существовавшие протоколы. Применение разработанного состава питательной среды позволит сократить материальные и трудовые затраты при реализации программ по сохранению ценных лекарственных ресурсов.
Keywords: microcloning, reproduction, rare species, conservation, cultivation conditions, Aristolochia, in vitro, микроклонирование, размножение, редкий вид, сохранение, условия культивирования
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